Cover of: High throughput protein expression and purification | edited by Sharon A. Doyle.

High throughput protein expression and purification

methods and protocols

Published by Humana Press, Springer, distributor] in Totowa, N.J, [London .
Written in English.

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Table of Contents

High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt
Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley
Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath
Flexi vector cloning / Paul G. Blommel ... [et al.]
The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens
The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley
A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.]
"System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart
Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery
E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh
PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood
High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina
High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.]
High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee
The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens
High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi
Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.]
Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh
High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery.

Edition Notes

Includes bibliographical references and index.

Methods in molecular biology -- 498, Methods in molecular biology (Clifton, N.J.) -- 498.


Dewey Decimal Class
Library of Congress
QP551 .H493 2009

The Physical Object

xii, 322 p. :
Number of pages

ID Numbers

Open Library
Internet Archive
1588298795, 1597451967
9781588298799, 9781597451963
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